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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
02/08/2016 |
Data da última atualização: |
28/03/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
VIOL, M. A.; GUERRERO, F. D.; OLIVEIRA, B. C. M. de; AQUINO, M. C. C. de; LOIOLA, S. H.; MELO, G. D. de; GOMES, A. H. de S.; KANAMURA, C. T.; GARCIA, M. V.; ANDREOTTI, R.; LIMA, V. M. F. de; BRESCIANI, K. D. S. |
Afiliação: |
Milena Araúz Viol, UNESP; Felix D. Guerrero, UNESP; Bruno César Miranda de Oliveira, UNESP; Monally Conceição Costa de Aquino, UNESP; Saulo Hudson Loiola, UNESP; Guilherme Dias de Melo, UNESP; Aparecida Helena de Souza Gomes, Instituto Adolfo Lutz; Cristina Takami Kanamura, INSTITUTO ADOLFO LUTZ; Marcos Valério Garcia, FUNDECT; RENATO ANDREOTTI E SILVA, CNPGC; Valéria Marçal Félix de Lima, UNESP; Katia Denise Saraiva Bresciani, UNESP. |
Título: |
Identification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Parasitology Research, v. 115, n, 9, p. 3479-3484, sept. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Sand flies are recognized as the major vector of canine visceral leishmaniasis. However, in some areas of Brazil where sand flies do not occur, this disease is found in humans and dogs. There has been speculation that ticks might play a role in transmission of canine visceral leishmaniasis and the DNA of Leishmania spp. has been reported in whole ticks. We investigated the presence of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus ticks collected from tickinfested dogs in two cities of Brazil. We used 66 dogs that tested positive and 33 that tested negative for Leishmania spp. according to direct cytological examination assays. Ten ticks were collected from each dog and dissected to collect the intestines, ovaries, and salivary glands for immunohistochemistry (IHC) and diagnostic real-time polymerase chain reaction (RT-PCR). IHC results showed Leishmania spp. in 98, 14, and 8 % of the intestines, ovaries, and salivary glands, respectively. Real-time PCR showed that 89, 41, and 33 % of the tick intestine, ovary, and salivary glands, respectively, were positive for Leishmania spp. The verification of promastigotes of Leishmania spp. by two independent techniques in ticks collected from these urban region dogs showed that there is need for clarification of the role of ticks in the transmission of canine visceral leishmaniasis in Brazil. |
Palavras-Chave: |
Ixodids. |
Thesagro: |
Cão; Carrapato; Leishmaniose. |
Thesaurus Nal: |
Dogs; Immunohistochemistry; Leishmaniasis; Quantitative polymerase chain reaction. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/158208/1/Identification-of-Leishmania-spp.-promastigotes.pdf
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Marc: |
LEADER 02476naa a2200349 a 4500 001 2050014 005 2017-03-28 008 2016 bl uuuu u00u1 u #d 100 1 $aVIOL, M. A. 245 $aIdentification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs.$h[electronic resource] 260 $c2016 520 $aSand flies are recognized as the major vector of canine visceral leishmaniasis. However, in some areas of Brazil where sand flies do not occur, this disease is found in humans and dogs. There has been speculation that ticks might play a role in transmission of canine visceral leishmaniasis and the DNA of Leishmania spp. has been reported in whole ticks. We investigated the presence of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus ticks collected from tickinfested dogs in two cities of Brazil. We used 66 dogs that tested positive and 33 that tested negative for Leishmania spp. according to direct cytological examination assays. Ten ticks were collected from each dog and dissected to collect the intestines, ovaries, and salivary glands for immunohistochemistry (IHC) and diagnostic real-time polymerase chain reaction (RT-PCR). IHC results showed Leishmania spp. in 98, 14, and 8 % of the intestines, ovaries, and salivary glands, respectively. Real-time PCR showed that 89, 41, and 33 % of the tick intestine, ovary, and salivary glands, respectively, were positive for Leishmania spp. The verification of promastigotes of Leishmania spp. by two independent techniques in ticks collected from these urban region dogs showed that there is need for clarification of the role of ticks in the transmission of canine visceral leishmaniasis in Brazil. 650 $aDogs 650 $aImmunohistochemistry 650 $aLeishmaniasis 650 $aQuantitative polymerase chain reaction 650 $aCão 650 $aCarrapato 650 $aLeishmaniose 653 $aIxodids 700 1 $aGUERRERO, F. D. 700 1 $aOLIVEIRA, B. C. M. de 700 1 $aAQUINO, M. C. C. de 700 1 $aLOIOLA, S. H. 700 1 $aMELO, G. D. de 700 1 $aGOMES, A. H. de S. 700 1 $aKANAMURA, C. T. 700 1 $aGARCIA, M. V. 700 1 $aANDREOTTI, R. 700 1 $aLIMA, V. M. F. de 700 1 $aBRESCIANI, K. D. S. 773 $tParasitology Research$gv. 115, n, 9, p. 3479-3484, sept. 2016.
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Embrapa Gado de Corte (CNPGC) |
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Registros recuperados : 19 | |
8. | | GUERRERO, F. D.; LEÓN, A. A. P. de; RODRIGUEZ- VIVAS, R. I.; JONSSON, N.; MILLER, R. J.; ANDREOTTI, R. Acaricide research and development, resistance, and resistance monitoring. In: SONENSHINE, D. E.; ROE, R. M. (Ed.). Biology of ticks. 2nd ed. New York: Oxford, 2014.Tipo: Capítulo em Livro Técnico-Científico |
Biblioteca(s): Embrapa Gado de Corte. |
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9. | | ANDREOTTI, R.; GUERRERO, F. D.; SOARES, M. A.; BARROS, J. C.; MILLER, R. J.; PÉREZ DE LEÓN, A. Acaricide resistance of Rhipicephalus (Boophilus) microplus in State of Mato Grosso do Sul, Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 20, n. 2, p. 127-133, Apr.-Jun. 2011.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Gado de Corte. |
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11. | | CUNHA, R. C.; PÉREZ DE LEÓN, A. A.; LEITE, F. P. L.; GUERRERO, F. D.; PINTO, L. S.; ANDREOTTI, R. Assessment of bovine immunoprotection against Rhipicephalus microplus using Bm86-CG antigen expressed in Pichia pastoris. In: AMERICAN ASSOCIATION OF VETERINARY PARASITOLOGISTS ANNUAL MEETING, 56.; LIVESTOCK INSECT WORKERS CONFERENCE, 55.; INTERNATIONAL SYMPOSIUM ON ECTOPARASITES OF PETS, 11.,2011,ST. LOUIS, MO. Sustainable parasite and vector control: proceedings. St. Louis,MO: American Association of Veterinary Parasitologists, 2011. p.95Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Corte. |
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12. | | SABATINI, G. A.; RIBOLLA, P. E. M.; BARROS, A. T. M. de; GUERRERO, F. D.; SHUMAKER, T. T. S. Knockdown resistance in pyrethroid-resistant horn fly (Diptera: Muscidae) populations in Brazil. Revista Brasileira Parasitologia Veterinária, Jaboticabal, v. 18; n.3, p.311-316, jul/set, 2009.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
Biblioteca(s): Embrapa Pantanal. |
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13. | | GUERRERO, F. D.; CAMERON, C.; FOIL, L. D.; BARROS, A. T. M. de; CROW, J.; RETZEL, E. Molecular sequence dataset to identify metabolic-based pyrethroid resistance in the horn fly, Haematobia irritans. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 17., 2012, São Luis. Parasitologia veterinária, bem estar e produção animal: anais. São Luis: Colégio Brasileiro de Parasitologia Veterinária, 2012. Resumo: PA 149. p. 193Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pantanal. |
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15. | | ANDREOTTI, R.; PEREZ DE LEON, A; GUERRERO, F. D.; BENDELE, K. E.; SCOLES, G. A.; DOWD, S. E. Symbiotic bacteria in the cattle tick rhipicephalus microplus. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Gado de Corte. |
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17. | | BRITO, L. G.; OLIVEIRA, M. C. de S.; FOIL, L. D.; GUERRERO, F. D.; LEON, A. P. de; BARBIERI, F. da S.; CHAGAS, A. C. de S.; BARROS, A. T. M. de. Characterization of pesticede resistance in horn fly populations as a risk for fixation fo restance alles in cattle tick population. In: SIMPÓSIO EMBRAPA LABEX EUA DE SANIDADE ANIMAL, 2., 2012, Brasília, DF. Anais... Brasília: Embrapa Estudos e Capacitação, 2012. p.129-130Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Pecuária Sudeste. |
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18. | | BRITO, L. G.; OLIVEIRA, M. C. de S.; FOIL, L. D.; GUERRERO, F. D.; PEREZ DE LEON, A.; BARBIERI, F. da S.; CHAGAS, A. C. de S.; BARROS, A. T. M. de. Characterization of pesticide in horn fly populations as a risk factor for fixation of resistance alleles in cattle tick populations. In: SIMPÓSIO EMBRAPA LABEX EUA DE SANIDADE ANIMAL, 2., Brasília, 2012. Proceedings... Brasília: Embrapa Estudos e Capacitação, 2012. p. 129Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Pantanal. |
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19. | | VIOL, M. A.; GUERRERO, F. D.; OLIVEIRA, B. C. M. de; AQUINO, M. C. C. de; LOIOLA, S. H.; MELO, G. D. de; GOMES, A. H. de S.; KANAMURA, C. T.; GARCIA, M. V.; ANDREOTTI, R.; LIMA, V. M. F. de; BRESCIANI, K. D. S. Identification of Leishmania spp. promastigotes in the intestines, ovaries, and salivary glands of Rhipicephalus sanguineus actively infesting dogs. Parasitology Research, v. 115, n, 9, p. 3479-3484, sept. 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Gado de Corte. |
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Registros recuperados : 19 | |
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